   2.3.1.211 | more |
construction of an artificial curcuminoid biosynthetic pathway, including reactions of phenylalanine ammonia-lyase from the yeast Rhodotorula rubra, 4-coumarate:CoA ligase from Lithospermum erythrorhizon and curcuminoid synthase from Oryza sativa in Escherichia coli for the production of curcuminoids. Cultivation of the recombinant Escherichia coli cells in the presence of tyrosine or phenylalanine, or both, leads to production of bisdemethoxycurcumin, dicinnamoylmethane and cinnamoyl-4-coumaroylmethane, overview. Another Escherichia coli system carrying the 4-coumarate:CoA ligase and the CUS genes is also useful for high-yield production of curcuminoids from exogenously supplemented phenylpropanoid acids 4-coumaric acid, cinnamic acid and ferulic acid |
694037 |
