EC Number |
Protein Variants |
Reference |
---|
2.3.1.169 | A110C |
absence of strong cooperative inhibition of CO which characterizes wild-type enzyme |
655801 |
2.3.1.169 | A110C |
mutant designed to block the CO-migrating tunnel in the alpha-subunit. Electron paramagnetic resonance spectra indicates that the A-cluster is properly assembled. ACS activity is similar to that of the wild-type recombinant Ni-activated alpha-subunit |
687824 |
2.3.1.169 | A219F |
mutant designed to block the tunnel through which CO and CO2 migrate. Metal clusters are properly assembled but only slowly reducible by CO. Mutant shows impaired ability of CO to migrate through the tunnel to the C-cluster and reduced catalytic activity, no cooperative CO inhibition is observed |
674925 |
2.3.1.169 | A222L |
mutant designed to block the CO-migrating tunnel in the alpha-subunit. Electron paramagnetic resonance spectra indicates that the A-cluster is properly assembled. ACS activity is similar to that of the wild-type recombinant Ni-activated alpha-subunit |
687824 |
2.3.1.169 | A222L |
the bifunctional mutant enzyme is not able to synthesize acetyl-CoA using CO2 as substrate |
655801 |
2.3.1.169 | A265M |
absence of strong cooperative inhibition of CO which characterizes wild-type enzyme |
655801 |
2.3.1.169 | A578C |
mutant designed to block the tunnel through which CO and CO2 migrate. Metal clusters are properly assembled but only slowly reducible by CO. Mutant shows impaired ability of CO to migrate through the tunnel to the C-cluster and reduced catalytic activity, no cooperative CO inhibition is observed |
674925 |
2.3.1.169 | F70W |
mutant designed to block the region that connects the tunnel at the betabeta interface with a water channel also located at the interface. Metal clusters are properly assembled but only slowly reducible by CO. Mutant shows impaired ability of CO to migrate through the tunnel to the C-cluster and reduced catalytic activity, no cooperative CO inhibition is observed |
674925 |
2.3.1.169 | L215F |
mutant designed to block the tunnel through which CO and CO2 migrate. Metal clusters are properly assembled but only slowly reducible by CO. Mutant shows impaired ability of CO to migrate through the tunnel to the C-cluster and reduced catalytic activity, no cooperative CO inhibition is observed |
674925 |
2.3.1.169 | more |
construction of several cdh gene knockouts, genotypic and phenotypic analysis of cdh mutants. CODH activity during aceticlastic and carboxidotrophic growth is reduced in the cdh1 mutant (strain MCD1) compared to the wild-type |
719744 |