EC Number   |
Protein Variants |
Reference |
|---|
   2.3.1.164 | C103S |
unprocessed inactive variant of isopenicillin N acyltransferase |
686918 |
| 2.3.1.164 | C103S or A or W |
C103 is specifically required for recombinant proenzyme cleavage |
441306 |
| 2.3.1.164 | more |
disruption of the aatA gene reduces penicillin production to 20%, the remaining activity is attributed to the product of gene aatB |
705776 |
| 2.3.1.164 | more |
in high-yielding penicillin production strains IAT is the limiting factor and that this limitation can be alleviated by a balanced overproduction of this enzyme. Cooverexpression of penDE with gene phl, encoding the phenylacetic acid CoA ligase leading to production of phenoxyacetyl-CoA and penicillin V |
-, 718653 |
| 2.3.1.164 | more |
Overexpression of a mutated aatB-PTS1 gene in an aatA-disruption strain, leading to peroxisomal localization of AatB, increased the penicillin titre more than overexpression of the wild-type aatB. |
705776 |
| 2.3.1.164 | S227A |
produces uncleaved proenzyme lacking enzyme activity |
441304 |
| 2.3.1.164 | S309A |
recombinant proenzyme containing S309A is cleaved, however enzyme activity is not observed |
441304 |
| 2.3.1.164 | T105V |
results in a significant inhibition of proenzyme cleavage |
441306 |
