EC Number   |
Protein Variants |
Reference |
|---|
   2.3.1.159 | more |
the starter substrates for chalcone synthase or acridone synthase reaction are placed in different topographies in the active site pocket of the enzymes. Therefore conformational changes in the periphery beyond the active site cavity volumes determine the product formation and interconversion of enzymes by mutation of the active sites is insufficient |
676366 |
| 2.3.1.159 | S132T/A133S/V265F |
site-directed mutagenesis, exchange of 3 residues, responsible for substrate activity, by the corresponding amino acids of chalcone synthase results in transformation of the enzyme to a chalcone synthase, 25fold increased activity, with only 36% remaining acridone synthase activity compared to wild-type |
486402 |
| 2.3.1.159 | V265F |
site-directed mutagenesis, 75% reduction of ACS catalytic activity, but 2fold increased chalcone synthase activity |
486402 |
