EC Number   |
Protein Variants |
Reference |
|---|
    2.1.3.1 | A87G |
Km not significantly changed, significantly reduced kcat |
485822 |
| 2.1.3.1 | M88A |
Km not significantly changed, significantly reduced kcat |
485822 |
| 2.1.3.1 | M88C |
Km not significantly changed, significantly reduced kcat |
485822 |
| 2.1.3.1 | M88L |
Km not significantly changed, significantly reduced kcat |
485822 |
| 2.1.3.1 | M88L |
partial reaction 1: 99% loss activity, partial reaction 2: 65% loss of activity, possibly alterations in the microenvironment of the biocytin |
485829 |
| 2.1.3.1 | M88T |
Km not significantly changed, significantly reduced kcat |
485822 |
| 2.1.3.1 | M90L |
Km and kcat not significantly changed |
485822 |
| 2.1.3.1 | M90L |
partial reaction 1: 50% loss of activity, partial reaction 2: 115% activity compared to the 1.3 wild-type enzyme |
485829 |
| 2.1.3.1 | more |
double mutant A87M and M88A |
485822 |
| 2.1.3.1 | more |
metabolic engineering of Propionibacteriumfreudenreichii subsp. shermanii DSM 4902 for enhanced propionic acid fermentation by overexpression of pyruvate carboxylase, methylmalonyl-CoA decarboxylase, and methylmalonyl-CoA carboxyltransferase, three biotin-dependent carboxylases in the dicarboxylic acid pathway controlling the carbon fluxin the WoodWerkman cycle, from Propionibacterium acidipropionici ATCC 4875 in Propionibacterium shermani. The co-fermentation kinetics show that with more glycerol as carbon source, both the wild-type and Ps(pKPYC4) produce more propionic acid and succinic acid, but acetic acid formation is not as significantly affected, neither is the Gly/Glu ratio, metabolic flux analysis, detailed overview |
-, 737181 |
