EC Number |
Protein Variants |
Reference |
---|
2.1.1.74 | C193A |
mutant is nearly as active as the wild-type enzyme |
719972 |
2.1.1.74 | C223A |
site-directed mutagenesis, almost inactive mutant |
706541 |
2.1.1.74 | C226A |
mutant loses both the tRNA methylation activity and the capacity to form a covalent complex with the 5-FU-mini-RNA |
719972 |
2.1.1.74 | C51A |
site-directed mutagenesis, almost inactive mutant |
706541 |
2.1.1.74 | C53A |
mutant is inactive but like the wild-type enzyme, mutant C53A is capable of forming a covalent complex with a 5-fluorouridine-containing mini-RNA. Mutation of Cys-53 changes the accessibility of the FAD-binding site and impairs the conformational stability of TrmFO |
719972 |
2.1.1.74 | C53A/C226A |
as for the single C226A mutant, no protein-RNA covalent complex is detectable with the double mutant |
719972 |
2.1.1.74 | E341A |
site-directed mutagenesis, the active mutant shows a decrease in both FAD binding and methylation activity compared to the wild-type enzyme |
706541 |
2.1.1.74 | H308A |
site-directed mutagenesis, the mutant shows 57% of wild-type enzyme activity compared to the wild-type enzyme |
706541 |
2.1.1.74 | K282A |
site-directed mutagenesis, almost inactive mutant |
706541 |
2.1.1.74 | K287A |
site-directed mutagenesis, almost inactive mutant |
706541 |