EC Number   |
Protein Variants |
Reference |
|---|
   2.1.1.63 | A121E |
no major structural change upon energy minimization, is within the DNA binding region and may therefore affect DNA binding of MGMT |
674061 |
| 2.1.1.63 | A121T |
no major structural change upon energy minimization, is within the DNA binding region and may therefore affect DNA binding of MGMT |
674061 |
| 2.1.1.63 | A154T |
ca. 4fold increased activity, AGT mutant selected using phage display |
676938 |
| 2.1.1.63 | A41D/S115T/I151N |
1.6fold increased activity, AGT mutant selected using yeast three-hybrid system |
676938 |
| 2.1.1.63 | C119F |
the mutant shows reduced thermal stability compared to the wild type enzyme |
-, 743372 |
| 2.1.1.63 | C119L |
the mutant shows reduced thermal stability compared to the wild type enzyme |
-, 743372 |
| 2.1.1.63 | C145A |
abolishes 1,2,3,4-diepoxybutane-induced cross-linking at this site, while the formation of conjugates via neighboring Cys150 is retained |
673065 |
| 2.1.1.63 | C145A |
binds to alkylated DNA in a similar manner to wild type AGT but is unable to carry out the repair transfer, expressed in Escherichia coli, this mutant increases killing and mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine |
673219 |
| 2.1.1.63 | C145A |
inactive mutant enzyme forms a specific and stable complex with a 6-O-methylguanine-containing oligonucleotide substrate |
485490 |
| 2.1.1.63 | C145A |
inactive mutant protein |
658445 |
