EC Number   |
Protein Variants |
Reference |
|---|
   2.1.1.35 | C324A |
level of total TrmA-associated peptide 41%, amount of m5U in total tRNA 4%, relative to the levels found in the wild-type |
689248 |
| 2.1.1.35 | C521A |
mutant without methyltransferase activity, putative nucleophilic Cys-521 may be involved in Cys-U54 bond formation |
441381 |
| 2.1.1.35 | D299A |
level of total TrmA-associated peptide 63%, amount of m5U in total tRNA 1%, relative to the levels found in the wild-type |
689248 |
| 2.1.1.35 | E358K |
level of total TrmA-associated peptide 59%, amount of m5U in total tRNA 9%, relative to the levels found in the wild-type |
689248 |
| 2.1.1.35 | E358Q |
mutation of the catalytic base Glu-358 to Gln arrests catalysis and allows isolation of the covalent protein-RNA complex for crystallization |
689809 |
| 2.1.1.35 | F188A |
level of total TrmA-associated peptide 84%, amount of m5U in total tRNA 22%, relative to the levels found in the wild-type |
689248 |
| 2.1.1.35 | G220D |
level of total TrmA-associated peptide 76%, amount of m5U in total tRNA 2%, relative to the levels found in the wild-type |
689248 |
| 2.1.1.35 | G360D |
level of total TrmA-associated peptide 53%, amount of m5U in total tRNA 1%, relative to the levels found in the wild-type |
689248 |
| 2.1.1.35 | G428D |
mutant without methyltransferase activity, G-428 is located at the presumed S-adenosyl-L-methionine binding site |
441381 |
| 2.1.1.35 | Q190A |
level of total TrmA-associated peptide 77%, amount of m5U in total tRNA 14%, relative to the levels found in the wild-type |
689248 |
