EC Number |
Protein Variants |
Reference |
---|
2.1.1.264 | D195A |
site-directed mutagenesis, the mutation does not affect the methylation activity |
720598 |
2.1.1.264 | D568A |
site-directed mutagenesis, the D568A mutation in the C-terminal domain does not rescue m7G2069 formation, but m2G2445 is efficiently formed in this mutant |
720598 |
2.1.1.264 | D597A |
site-directed mutagenesis, the mutation does not affect the methylation activity |
720598 |
2.1.1.264 | more |
generation of a ycbY knock-out strain, completely inactive in methylation, from Escherichia coli strain BW25113 and complementation with active ycbY |
-, 720601 |
2.1.1.264 | N309A |
site-directed mutagenesis, the N309A mutation in the NTD impairs m2G2445 formation, but rescues m7G2069 formation |
720598 |
2.1.1.264 | N397A |
site-directed mutaagenesis, the N397A mutant exhibits no m2G2445 formation but rescues m7G2069 formation |
720598 |
2.1.1.264 | R530A |
site-directed mutagenesis, the mutation does not affect the methylation activity |
720598 |