EC Number   |
Protein Variants |
Reference |
|---|
   2.1.1.260 | D86G |
the mutation causes the Bowen-Conradi syndrome |
720582 |
| 2.1.1.260 | D90G |
a mutation in yeast Nep1 equivalent to the Bowen-Conradi syndrome, BCS, mutation in the human Nep1. Nep1 methyltransferase activity is not affected upon introduction of the BCS mutation, the mutated protein shows enhanced dimerization propensity and increased affinity for its RNA-target in vitro |
720587 |
| 2.1.1.260 | more |
complementation of a ScDnep1 strain with the human Nep1 (C2F) protein, the HsNEP1 open reading frame is expressed with the yeast inducible/repressible GAL1 promoter and the resulting plasmid (pGALHsNEP1) is transformed into the heterozygous ScDnep1/ScNEP1 strain CEN.SR679. After sporulation and tetrad analysis, ScDnep1 segregants complemented by the pGAL-HsNEP1 are only viable with galactose |
-, 719322 |
| 2.1.1.260 | more |
construction of a heterozygous CaDnep1/CaNEP1 strain CAE8 after replacement of one CaNEP1 wild-type allele with a CaURA3 marker and introduction of GFP-open reading frame driven by the methionine/cysteine-repressible CaMET3 promoter in front of the gene. Without high concentrations of methionine and cysteine in the medium, the resulting strain is viable, but addition of 2.5 mM methionine and 2.5 mM cysteine strongly impairs growth |
-, 719322 |
| 2.1.1.260 | more |
construction of a heterozygous CaDnep1/CaNEP1 strain CAE8 after replacement of one CaNEP1 wild-type allele with a CaURA3 marker and introduction of GFP-open reading frame driven by the methionine/cysteine-repressible CaMET3 promoter in front of the gene. Without high concentrationsof methionine and cysteine in the medium, the resulting strain is viable, but addition of 2.5 mM methionine and 2.5 mM cysteine strongly impairs growth |
-, 719322 |
| 2.1.1.260 | more |
lethal phenotype of a DELTAnep1 deletion, overexpression of RPS19B and also deletions within genes DELTAnop6 and DELTAtma23, respectively, which both encode small, positively charged fungal-specific proteins, suppress the DELTAnep1 growth deficiency. Identification of DELTanep1-specific genetic interactions, polysome profiles and phenotypes, overview. DELTAAutp30 enhances the phenotype of DELTAnep1 and is a multi-copy suppressor |
721118 |
