EC Number   |
Protein Variants |
Reference |
|---|
   2.1.1.244 | D168X |
site-directed mutagenesis, the mutation has no effect on methylation |
718160 |
| 2.1.1.244 | D178A/D181A |
site-directed mutagenesis, mutating the residues Asp178 and Asp181 at the lip of the active site to Ala decreases enzyme activity, which is further decreased by reverse-charge mutagenesis to Lys |
718160 |
| 2.1.1.244 | D180K |
site-directed mutagenesis, inactive mutant |
736186 |
| 2.1.1.244 | D180N |
site-directed mutagenesis, the mutant shows highly reduced activity compared to wild-type |
756877 |
| 2.1.1.244 | D180Y |
site-directed mutagenesis, inactive mutant |
736186 |
| 2.1.1.244 | D212N |
site-directed mutagenesis, the mutant shows reduced activity compared to wild-type |
756877 |
| 2.1.1.244 | E213A |
site-directed mutagenesis, the mutant shows 15% reduced activity compared to wild-type |
756877 |
| 2.1.1.244 | H140A |
the mutant loses the catalytic activity, but retains binding affinity to the peptide substrate |
756463 |
| 2.1.1.244 | H140K |
site-directed mutagenesis, inactive mutant |
736186 |
| 2.1.1.244 | more |
depleting NRMT in 293LT cells, using lentivirus, significantly decreases methylation of endogenous RCC1, while not affecting overall RCC1 level, depleting NRMT in HeLa cells using short interfering RNAs causing the same effects, RCC1 methylation is rescued by expression of murine NRMT-FLAG, which is not targeted by the human shRNA |
718160 |
