EC Number |
Protein Variants |
Reference |
---|
2.1.1.200 | E225A |
site-directed mutagenesis, inactive mutant |
-, 736907 |
2.1.1.200 | F199A |
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme |
-, 736907 |
2.1.1.200 | G231A |
site-directed mutagenesis, inactive mutant |
-, 736907 |
2.1.1.200 | I228A |
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme |
736907 |
2.1.1.200 | L229A |
site-directed mutagenesis, inactive mutant |
-, 736907 |
2.1.1.200 | more |
generation of several partial deletion mutants of TrmJ, including single site mutants, DELTA2 (deleted residues 171-172), DELTA4 (deleted residues 171-174), DELTA7 (deleted residues 169-175), DELTA10 (deleted residues 167-176), DELTA12 (deleted residues 166-177), NTD (residues 1-170) and CTD (residues 171-246) |
-, 736907 |