EC Number   |
Protein Variants |
Reference |
|---|
  2.1.1.182 | C168A |
site-directed mutagenesis, the mutation does not affect KsgA activity |
718932 |
| 2.1.1.182 | E43A |
mutation located in the S-adenosylmethionine-binding motifs severely reduces methyltransferase activity, the mutation retains the ability to suppress the growth defect of the Era(E200K) strain at a low temperature |
704274 |
| 2.1.1.182 | E66A |
catalytically inactive enzyme form |
705774 |
| 2.1.1.182 | E66A |
mutation located in the S-adenosylmethionine-binding motifs severely reduces methyltransferase activity, the mutation retains the ability to suppress the growth defect of the Era(E200K) strain at a low temperature |
704274 |
| 2.1.1.182 | G47A |
mutation located in the S-adenosylmethionine-binding motifs severely reduces methyltransferase activity, the mutation retains the ability to suppress the growth defect of the Era(E200K) strain at a low temperature |
704274 |
| 2.1.1.182 | L114P |
site-directed mutagenesis of the active site residue, the KsgA mutant shows diminished overall activity, and impaired ability to methylate the N6-methyladenosine intermediate to produce N6,N6-dimethyladenosine. Reduced activity is not due to disruption of 30S substrate binding |
718932 |
| 2.1.1.182 | N113A |
site-directed mutagenesis of the active site residue, the KsgA mutant shows diminishes activity to a level comparable to L114P without affecting the methylation of N6-methyladenosine. Reduced activity is not due to disruption of 30S substrate binding |
718932 |
| 2.1.1.182 | R248A |
mutation at the C-terminal does not affect the methyltransferase activity and fails to suppress the growth defect of the Era(E200K) strain |
704274 |
