EC Number   |
Protein Variants |
Reference |
|---|
   2.1.1.170 | D56A |
site-directed mutagenesis of the catalytic residue, the mutant is streptomycin-resistant and shows reduced activity compared to the wild-type enzyme |
721042 |
| 2.1.1.170 | D71A |
site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-resistant and catalytically inactive |
721042 |
| 2.1.1.170 | D96A |
site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-resistant and catalytically inactive |
721042 |
| 2.1.1.170 | G73A |
site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-sensitive and shows reduced activity compared to the wild-type enzyme |
721042 |
| 2.1.1.170 | G75A |
site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-resistant and shows reduced activity compared to the wild-type enzyme |
721042 |
| 2.1.1.170 | G75A |
the mutation directly affects the S-adenosyl-L-methionine binding site, presents an almost 4fold loss of affinity and maintains the enzyme active |
733472 |
| 2.1.1.170 | G77A |
site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-resistant and catalytically inactive |
721042 |
| 2.1.1.170 | H53A |
site-directed mutagenesis of the catalytic residue, the mutant is partly streptomycin-resistant and shows reduced activity compared to the wild-type enzyme |
721042 |
| 2.1.1.170 | K100A/R101A |
site-directed mutagenesis of the RNA-binding residues, the mutant is streptomycin-resistant and catalytically inactive |
721042 |
| 2.1.1.170 | K165A |
site-directed mutagenesis of the RNA binding residue, the mutant is streptomycin-sensitive, but shows reduced activity compared to the wild-type enzyme |
721042 |
