EC Number |
Protein Variants |
Reference |
---|
2.1.1.169 | D263E |
severe loss of activity is due to a conflict between the catalytic His262-imidazole group and Glu-CH2 |
719183 |
2.1.1.169 | D263E |
site-directed mutagenesis, severe loss of activity is due to a conflict between the catalytic His262-imidazole group and Glu-CH2 |
719183 |
2.1.1.169 | D263I |
no activity, Ile263 can not form a H-bond with 3'-OH group |
719183 |
2.1.1.169 | D263I |
site-directed mutagenesis, Ile263 can not form a H-bond with 3'-OH group, the mutant shows almost complete loss in activity |
719183 |
2.1.1.169 | D263N |
site-directed mutagenesis, slight decrease in activity due to a decreased electronegativity of Asn-N compared to Asp-O, that affects charge transfer to tricetin-OH groups |
719183 |
2.1.1.169 | D263N |
slight decrease in activity due to a decreased electronegativity of Asn-N compared to Asp-O, that affects charge transfer to tricetin-OH groups |
719183 |
2.1.1.169 | E290I |
loss of activity is due to the fact that Ile can not form a H-bond with the 4'-OH of tricetin |
719183 |
2.1.1.169 | E290I |
site-directed mutagenesis, almost complete loss of activity is due to the fact that Ile can not form a H-bond with the 4'-OH of tricetin |
719183 |
2.1.1.169 | E290Q |
no activity. This mutation results in a more extensive H-bonding that hinders charge transfer and affects B-ring flexibility |
719183 |
2.1.1.169 | E290Q |
site-directed mutagenesis, the mutation results in a more extensive H-bonding that hinders charge transfer and affects B-ring flexibility and almost complete loss in activity |
719183 |