EC Number   |
Protein Variants |
Reference |
|---|
    2.1.1.104 | D168L |
the mutation abolishes the enzyme activity of OMT-15 |
700855 |
| 2.1.1.104 | D194L |
the mutation abolishes the enzyme activity of OMT-15 |
700855 |
| 2.1.1.104 | D209L |
the mutation abolishes the enzyme activity of OMT-17 |
700855 |
| 2.1.1.104 | D228A |
only residual enzyme activities are detectable |
734862 |
| 2.1.1.104 | D234L |
the mutation abolishes the enzyme activity of OMT-17 |
700855 |
| 2.1.1.104 | D252A |
inactive |
758038 |
| 2.1.1.104 | D58A |
decrease of catalytic activity |
485066 |
| 2.1.1.104 | E69L |
the mutation results in about 14% loss of enzyme activity in OMT-15 and 40% loss of activity in OMT-17 |
700855 |
| 2.1.1.104 | G46Y |
mutation is sufficient for a reversal of the unusual para- back to meta-O-methylation of flavanones and dihydroflavonols |
733869 |
| 2.1.1.104 | G46Y/W193F/R194M/I195K |
methylation in meta-position is preferred, mutations reduce the affinity for eriodictyol due to almost no interactions of the side chains with the substrate |
733869 |
