EC Number   |
Protein Variants |
Reference |
|---|
  1.8.5.9 | C104S |
inactive |
756872 |
| 1.8.5.9 | C130S |
inactive |
756717 |
| 1.8.5.9 | C41A/C44A |
the mutant has two cysteine residues at Cys104 and Cys130 compared to the wild type enzyme with Cys41, Cys44, Cys104 and Cys130 |
757146 |
| 1.8.5.9 | C41S/C44S/C102S/C130S |
inactive |
-, 756909 |
| 1.8.5.9 | more |
construction of mutant CtDsbB-CCSS, in which periplasmic loop 2 Cys98 and Cys104 are mutated to serines. In the presence of CtDsbB-CCSS or CtDsbB-SSCC, CtDsbA catalysed oxidation of the peptide substrate is markedly reduced relative to wild-type CtDsbB, although oxidation proceeds more rapidly than observed for negative controls containing only buffer, or the wild-type CtDsbB variant alone. The disulfide bonds present in periplasmic loops P1 and P2 of CtDsbB are each required for complete oxidation of CtDsbA |
-, 758183 |
| 1.8.5.9 | R118H |
missense mutation |
-, 756909 |
| 1.8.5.9 | R48A |
the mutant shows strongly reduced DsbA protein oxidation activity |
758211 |
| 1.8.5.9 | R48H |
the mutant shows reduced activity compared to the wild type enzyme |
756585 |
| 1.8.5.9 | R48H |
the mutant shows reduced affinity for ubiquinone |
758288 |
| 1.8.5.9 | R48H |
the mutant shows strongly reduced DsbA protein oxidation activity |
758211 |
