EC Number   |
Protein Variants |
Reference |
|---|
   1.8.5.1 | A140D |
no alteration in specific activity compared to the wild type enzyme |
670490 |
| 1.8.5.1 | C22A |
site-directed mutagenesis, the mutation results in severe loss of the enzyme's function |
765604 |
| 1.8.5.1 | C23S |
mutant enzyme has almost no activity |
658634 |
| 1.8.5.1 | C25S |
has equivalent specificity constants for dehydroascorbate and GSH, but may have a different catalytic mechanism |
394032 |
| 1.8.5.1 | C26S |
turnover number is 57% of the wild-type value, KM-value for dehydroascorbate is 2fold lower than the wild-type value, KM-value for GSH is 1.6old lower than the wild-type value |
658634 |
| 1.8.5.1 | C29A |
site-directed mutagenesis, the mutant shows 8.1fold lower activity with dehydroascorbate compared to wild-type |
764040 |
| 1.8.5.1 | C9S |
turnover number is 86% of the wild-type value, KM-value for dehydroascorbate is 2.8fold lower than the wild-type value, KM-value for GSH is 2fold lower than the wild-type value |
658634 |
| 1.8.5.1 | C9S/C26S |
turnover number is 43% of the wild-type value, KM-value for dehydroascorbate is 1.1fold higher than the wild-type value, KM-value for GSH is identical to the wild-type value |
658634 |
| 1.8.5.1 | D21A |
site-directed mutagenesis, the mutation results in severe loss of the enzyme's function |
765604 |
| 1.8.5.1 | D21N |
site-directed mutagenesis, the mutation results in severe loss of the enzyme's function |
765604 |
