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EC Number Protein Variants Commentary Reference
Display the reaction diagram Show all sequences 1.8.4.15C21S site-directed mutagenesis, almost inactive mutant -, 763778
Display the reaction diagram Show all sequences 1.8.4.15C24S site-directed mutagenesis, the mutant shows increased activity compared to wild-type enzyme with a mixed disulfide substrate 763778
Display the reaction diagram Show all sequences 1.8.4.15more ncgl2478 gene in-frame deletion increasing the size of growth inhibition zones. Site-directed mutagenesis confirms Cys24 as the resolving Cys residue, while Cys21 is the nucleophilic cysteine that is oxidized to a sulfenic acid and then forms an intramolecular disulfide bond with Cys24 or a mixed disulfide with MSH under oxidative stress -, 763778
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