EC Number   |
Protein Variants |
Reference |
|---|
    1.5.8.2 | C-terminal truncations |
C-terminal truncations of 5, 10 or 17 residues do not affect dimer stability, but compromise the ability of the enzyme to become covalently coupled with FMN in the active site |
-, 393892 |
| 1.5.8.2 | C30A |
binds FMN non-covalently |
-, 393888, 393897 |
| 1.5.8.2 | C30A |
mutant shows highly increased activity to oxidate of the cofactor FMN to 6-hydroxyFMN compared to the wild-type enzyme |
654971 |
| 1.5.8.2 | C30A |
removal of the 6-S-cysteinyl-FMN bond, diminishes limiting rate for the first of three observed kinetic phases |
-, 393886 |
| 1.5.8.2 | H172Q |
no change of turnover, but 25fold increase of Km-value |
-, 393893 |
| 1.5.8.2 | V344I |
increase of Km-value, decrease of turnover |
-, 393895 |
| 1.5.8.2 | Y442F |
- |
-, 393898 |
| 1.5.8.2 | Y442F |
1.4fold reduction of electron transfer |
-, 393891 |
| 1.5.8.2 | Y442G |
- |
-, 393895, 393898 |
| 1.5.8.2 | Y442G |
30.5fold reduction of electron transfer |
-, 393891 |
