EC Number   |
Protein Variants |
Reference |
|---|
  1.5.3.25 | C324A |
mutation of putative active site residue, no residual activity with fructosyl L-valine |
764360 |
| 1.5.3.25 | C342A |
about 2% of wild-type activity, loss of covalent binding of FAD |
764126 |
| 1.5.3.25 | C342S |
less than 1% of wild-type activity, loss of covalnet binding of FAD |
764126 |
| 1.5.3.25 | D295C/K303C |
mutation leads to increase in melting temperature, mutant shows hyperstabilization with residual activity up to 95°C |
765813 |
| 1.5.3.25 | E280L |
mutation in fructosyl binding site, loss of activity |
765037 |
| 1.5.3.25 | E71R |
activity similar to wild-type |
765037 |
| 1.5.3.25 | E71R/R114E |
8fold increase in Km value |
765037 |
| 1.5.3.25 | E71S |
mutation in salt bridge between flexible regions, activity similar to wild-type |
765037 |
| 1.5.3.25 | E75N/R108L7R114W/W208R/Q289R/T303S |
8.78fold increase in the activity toward fructosyl-polylysine and also performs several fold better than the wild-type on real gravy stains at concentrations of 10-100 microg/ml |
764003 |
| 1.5.3.25 | F356A |
mutation of putative active site residue, 18% of wild-type activity with fructosyl L-valine |
764360 |
