EC Number |
Protein Variants |
Reference |
---|
1.5.3.24 | C141S |
the mutant exhibits specific activity values reduced to those of the wild type recombinant enzyme or the natural enzyme |
764129 |
1.5.3.24 | C195S |
the mutant exhibits specific activity values similar to those of the wild type recombinant enzyme or the natural enzyme |
764129 |
1.5.3.24 | C351S |
the mutant exhibits specific activity values similar to those of the wild type recombinant enzyme or the natural enzyme |
764129 |
1.5.3.24 | G139A |
the mutant of the alpha subunit is expressed only at low temperature (15°C), but the purified enzyme exhibits properties indistinguishable from the wild type enzyme |
764129 |
1.5.3.24 | G30A |
the mutant of the beta subunit exhibits subunit expression levels similar to those of the wild type enzyme, but the mutation blocks subunit assembly and covalent attachment of FMN |
764129 |
1.5.3.24 | H173N |
catalytically inactive mutant of the beta subunit |
764129 |
1.5.3.24 | H175A |
the mutant contains 1 mol of covalently bound flavin (FMN) and exhibits catalytic activity similar to the wild type enzyme |
764124 |
1.5.3.24 | K171A |
the mutant shows 39% activity of the wild type enzyme |
765017 |
1.5.3.24 | K171D |
the mutant shows 32% activity of the wild type enzyme |
765017 |
1.5.3.24 | K171R |
the mutant shows 58% activity of the wild type enzyme |
765017 |