EC Number |
Protein Variants |
Reference |
---|
1.5.3.1 | C265A |
almost wild type activity but much more stable against thiol modifying inhibitors |
392404 |
1.5.3.1 | C265D |
almost wild type activity but much more stable against thiol modifying inhibitors |
392404 |
1.5.3.1 | C265R |
almost wild type activity but much more stable against thiol modifying inhibitors |
392404 |
1.5.3.1 | C265S |
almost wild type activity but much more stable against thiol modifying inhibitors |
392404 |
1.5.3.1 | C314A |
mutation of the covalent attachment site of FAD, mutant forms unstable complexes with FAD. In situ reconstitution of activity by assaying mutant in presence of FAD or 8-nor-8-chloro-FAD, giving a specific activity of 14% or 80% of wild-type, resp. Mutant exhibits high affinity for reduced flavin |
672210 |
1.5.3.1 | C315A |
inactive, no bound FAD, forms stable non-covalent complex with 5-deazaFAD |
685194 |
1.5.3.1 | C318S |
modification of covalent flavin attachment site, mutant with extremely weak activity |
392399 |
1.5.3.1 | C318S |
no enzymatic activity |
392404 |
1.5.3.1 | D35A |
D35 important for interaction with FAD |
392403 |
1.5.3.1 | D35E |
D35 important for interaction with FAD |
392403 |