EC Number |
Protein Variants |
Reference |
---|
1.5.1.6 | C152A |
95% of the activity with wild-type enzyme |
652526 |
1.5.1.6 | C17A |
94% of the activity with wild-type enzyme |
652526 |
1.5.1.6 | C191A |
96% of the activity with wild-type enzyme |
652526 |
1.5.1.6 | C238A |
102% of the activity with wild-type enzyme |
652526 |
1.5.1.6 | C707A |
- |
392350 |
1.5.1.6 | C707A |
catalytically inactive mutant |
392350 |
1.5.1.6 | C707A |
mutant with a complete loss of dehydrogenase activity of 10-formyltetrahydrofolate and propanal, but hydrolase activity and binding of NADP+ is unchanged |
392353 |
1.5.1.6 | C707A |
mutant with a complete loss of NADP+-dependent 10-formyltetrahydrofolate dehydrogenase activity, but no influence on hydrolase activity |
392342 |
1.5.1.6 | C707A |
site-directed mutagenesis of the C-terminal residue, replacement of Cys707 with an alanine results in the enzyme lacking the ability to differentiate between the oxidized and reduced coenzyme |
742257 |
1.5.1.6 | C86A |
110% of the activity with wild-type enzyme |
652526 |