EC Number   |
Protein Variants |
Reference |
|---|
   1.4.3.19 | A45H/G300C |
monomeric in solution. Upon incubation of apoprotein with FAD, neither FAD binding nor enzymatic activity is observed. Slow elimination of urea from partially unfolded mutant in presence of a large excess of FAD and 30% glyccerol does not produce the holoenzyme |
696489 |
| 1.4.3.19 | A54E |
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme |
742488 |
| 1.4.3.19 | A54R |
20fold increase in Km for glyphosate |
698957 |
| 1.4.3.19 | A54R |
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme |
-, 742488 |
| 1.4.3.19 | A54R/H244K |
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme |
742488 |
| 1.4.3.19 | A54R/H244K/M261R |
the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme |
742488 |
| 1.4.3.19 | agriculture |
expression of an evolved engineered variant of glycine oxidase leads to glyphosate resistance in alfalfa |
-, 742945 |
| 1.4.3.19 | BG51R/G60S/I198V/E357G |
the mutant shows no activity with glycine and high activity with glyphosate |
743639 |
| 1.4.3.19 | BG51R/G60S/S210P/M267T |
the mutant shows no activity with glycine and activity with glyphosate |
743639 |
| 1.4.3.19 | D103Y/D121G/S122P/R238K/K367R/T368R |
mutant displays increased activity towards glyphosate with dramatic loss of activity with glycine |
763884 |
