EC Number   |
Protein Variants |
Reference |
|---|
    1.3.98.1 | C130A |
low activity with dihydroorotate, increasing activity above pH 8.0 with dihydrooxonate |
390913 |
| 1.3.98.1 | C130A |
the mutant enzyme exhibits binding affinities for dihydroorotate similar to that of the wild type enzyme, reduction is extremely slow compared to that of the wild type, the rate of reduction increases with pH showing no sign of a plateau |
685146 |
| 1.3.98.1 | C130A |
the mutant forms charge-transfer complexes upon binding 3,4-dihydroxybenzoate, but the maximum of the broad charge-transfer bands is shifted to 590 nm |
685158 |
| 1.3.98.1 | C130S |
the mutant enzyme exhibits binding affinities for dihydroorotate similar to that of the wild type enzyme, reduction is extremely slow compared to that of the wild type, the rate of reduction increases with pH showing no sign of a plateau |
685146 |
| 1.3.98.1 | C130S |
the mutant forms charge-transfer complexes upon binding 3,4-dihydroxybenzoate, but the maximum of the broad charge-transfer bands is shifted to 610 nm |
685158 |
| 1.3.98.1 | E206/K296E |
conversion of intermolecular salt bridge, mutant is fully active in concentrated solutions and dissociates into monomers upon dilution like wild-type enzyme |
657291 |
| 1.3.98.1 | E206A |
disturbance of intermolecular salt bridge, mutant retains almost complete activity |
657291 |
| 1.3.98.1 | E206K |
disturbance of intermolecular salt bridge, mutant activity is severely impaired |
657291 |
| 1.3.98.1 | H185A |
4fold increase in KM-value of CoQD, 50% increase in KM-value of L-dihydroorotate |
656285 |
| 1.3.98.1 | K136E |
little changes in activity |
656148 |
