EC Number |
Protein Variants |
Reference |
---|
1.3.1.34 | C334A/C337A |
the mutation exhibits 0.02% of wild type activity |
685196 |
1.3.1.34 | C337A |
the mutation results in the loss of most of the iron and acid-labile sulfur and exhibits 0.09% of wild type activity |
685196 |
1.3.1.34 | D137A |
relative activity: 3.32% (substrate: 2,4-decadienoyl CoA), 1.27% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%) |
725482 |
1.3.1.34 | D155A |
relative activity: 36.3% (substrate: 2,4-decadienoyl CoA), 59.6% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%) |
725482 |
1.3.1.34 | D186A |
relative activity: 6.21% (substrate: 2,4-decadienoyl CoA), 1.86% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%) |
725482 |
1.3.1.34 | D268A |
relative activity: 2.75% (substrate: 2,4-decadienoyl CoA), 1.14% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%) |
725482 |
1.3.1.34 | D86A |
relative activity: 1.70% (substrate: 2,4-decadienoyl CoA), 1.76% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%) |
725482 |
1.3.1.34 | E164A |
mutant exhibits 1.5% of the wild type activity |
685196 |
1.3.1.34 | E215A |
relative activity: 28.9% (substrate: 2,4-decadienoyl CoA), 7.59% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%) |
725482 |
1.3.1.34 | H252A |
the mutation exhibits 0.09% of wild type activity |
685196 |