EC Number   |
Protein Variants |
Reference |
|---|
    1.3.1.12 | DELTA1-19 |
delta19PD, more sensitive to temperature effects yielding a half-life of 55 min at 95°C versus 2 h for PD |
677010 |
| 1.3.1.12 | H131A |
chorismate mutase-prephenate dehydrogenase bifunctional enzyme, mutant enzyme retains 10% of dehydrogenase activity and 30% mutase activity compared to the wild-type enzyme, His131 is not an essential residue whose protonation state is critical for catalysis or substrate binding |
390552 |
| 1.3.1.12 | H147N |
inactive, binds prephenate with apparent affinity similar to wild-type |
698889 |
| 1.3.1.12 | H189N |
chorismate mutase-prephenate dehydrogenase bifunctional enzyme, mutant enzyme shows less than 0.01% the activity of wild-type mutase and dehydrogenase |
390552 |
| 1.3.1.12 | H197N |
chorismate mutase-prephenate dehydrogenase bifunctional enzyme, mutant enzyme exhibits no significant dehydrogenase activity, retains nearly wild-type mutase activity and unaltered Michaelis constants for chorismate, prephenate and NAD+ |
390552 |
| 1.3.1.12 | H217A |
40fold increase in Km for prephenate, no inhibition by tyrosine |
698889 |
| 1.3.1.12 | H217N |
30fold increase in Km for prephenate, no inhibition by tyrosine |
698889 |
| 1.3.1.12 | K37A |
chorismate mutase-prephenate dehydrogenase bifunctional enzyme, mutant protein poorly expressed, no detectable mutase or dehydrogenase activity, structural changes may result in its inactivity and instability |
390552 |
| 1.3.1.12 | K37Q |
chorismate mutase-prephenate dehydrogenase bifunctional enzyme, mutant enzyme exhibits no mutase activity while retaining wild-type dehydrogenase activity |
390552 |
| 1.3.1.12 | more |
delta19-PDH, delta25-PDH, and delta28-PDH, which produce stable and soluble proteins, delta36-PDH, delta52-PDH, and delta55-PDH are unstable |
674545 |
