EC Number |
Protein Variants |
Reference |
---|
1.2.4.4 | D108N |
less thermostable than wild type enzyme |
687543 |
1.2.4.4 | D200A |
less thermostable than wild type enzyme |
687543 |
1.2.4.4 | D200A/D108N |
the mutant is defective in folding and assembly and as no detectable overall activity |
687543 |
1.2.4.4 | D200N |
less thermostable than wild type enzyme |
687543 |
1.2.4.4 | D295A |
site-directed mutagenesis of an alpha-subunit residue of component E1b, increased activity compared to the wild-type |
656230 |
1.2.4.4 | D296A |
inactive enzyme, no ability of component E1 apoenzyme to reconstitute with thiamine diphosphate |
349090 |
1.2.4.4 | E198Q/D200N |
the mutant is defective in folding and assembly and as no detectable overall activity |
687543 |
1.2.4.4 | F364C |
E1alpha-missense mutation in type IA maple syrup urine disease that causes the occurence of exclusively alpha,beta dimers |
349091 |
1.2.4.4 | H146A |
site-directed mutagenesis, mutation of a beta'-subunit residue, no reductive acylation activity, low decarboxylation activity |
656171 |
1.2.4.4 | H147N |
site-directed mutagenesis, mutation of a beta'-subunit residue, no reductive acylation activity, low decarboxylation activity |
656171 |