EC Number |
Protein Variants |
Reference |
---|
1.2.1.79 | C262A |
active site mutation, nonfunctional because Cys-262 acts as a nucleophilel |
725523 |
1.2.1.79 | C262A |
mutation abolishes catalytic activity, catalytic residue |
725838 |
1.2.1.79 | C262A |
site-directed mutagenesis, Sp2771 mutant structure analysis and comparison to the wild-type structure |
743181 |
1.2.1.79 | C289A |
site-directed mutagenesis, inactive mutant |
763015 |
1.2.1.79 | C291A |
site-directed mutagenesis, the mutant shows 65% activity compared to wild-type enzyme |
763015 |
1.2.1.79 | E228A |
active site mutation, nonfunctional because Glu-228 acts as a general base |
725523 |
1.2.1.79 | E228A |
mutation abolishes catalytic activity, catalytic residue |
725838 |
1.2.1.79 | E228A |
site-directed mutagenesis, inactive mutant |
763137 |
1.2.1.79 | E228D |
sitedirected mutagenesis, the mutant shows highly reduced activity compared to wild-type enzyme |
763137 |
1.2.1.79 | E228Q |
active site mutation, nonfunctional because Glu-228 acts as a general base |
725523 |