EC Number   |
Protein Variants |
Reference |
|---|
   1.2.1.36 | C301A |
site-directed mutagenesis, catalytic residue mutation, reduced activity compared to the wild-type enzyme |
656252 |
| 1.2.1.36 | C302A |
site-directed mutagenesis of catalytic active center residue, the mutant ALDH1A1 loses both activities |
763529 |
| 1.2.1.36 | C303A |
site-directed mutagenesis of catalytic active center residue, the mutant ALDH1A1 loses both activities |
763529 |
| 1.2.1.36 | C456A |
site-directed mutagenesis of a cysteine residue outside the active center, the mutation has no effect on both the aldehyde-dehydrogenase activity and the GSH/DHLA-dependent NAD+-reduction activity |
763529 |
| 1.2.1.36 | E107G |
site-directed mutagenesis, mutation of a residue that contributes to conformational integrity, the mutant loses its aldehyde-dehydrogenase activity, yet completely retains its GSH/DHLA-dependent NAD+-reduction activity |
763529 |
| 1.2.1.36 | F471L |
mutant |
671851 |
| 1.2.1.36 | G246A/G251A |
site-directed mutagenesis of NAD+-binding sites, the mutant ALDH1A1 loses both the aldehyde-dehydrogenase activity and the GSH/DHLA-dependent NAD+-reduction activity |
763529 |
| 1.2.1.36 | I304K |
site-directed mutagenesis, the mutant loses its aldehyde-dehydrogenase activity |
763529 |
| 1.2.1.36 | K127A |
site-directed mutagenesis, the mutant shows reduced activity with retinoic acid compared to the wild-type enzyme |
742258 |
| 1.2.1.36 | K127A |
the mutant shows reduced activity compared to the wild type enzyme |
724722 |
