EC Number   |
Protein Variants |
Reference |
|---|
   1.14.99.48 | F23A |
mutation does not significantly affect heme binding or degradation |
725367 |
| 1.14.99.48 | H77A |
mutation abolishes IsdG-mediated heme degradation |
-, 725367 |
| 1.14.99.48 | L17A |
mutation does not significantly affect heme binding or degradation |
-, 725367 |
| 1.14.99.48 | M38A |
slight reduction of heme degradation, mutation shifts the peak absorbance of the Soret band to 430 nm |
725367 |
| 1.14.99.48 | N7A |
inactive |
725384 |
| 1.14.99.48 | N7A |
mutation abolishes IsdG-mediated heme degradation |
-, 725367 |
| 1.14.99.48 | S70A |
mutation does not significantly affect heme binding or degradation |
-, 725367 |
| 1.14.99.48 | W66A |
Trp66 is a conserved heme pocket residue implicated in heme ruffling. Mutant is inactive |
-, 740703 |
| 1.14.99.48 | W66F |
Trp66 is a conserved heme pocket residue implicated in heme ruffling. Mutant shows about half of wild-type heme degradation activity |
-, 740703 |
| 1.14.99.48 | W66L |
Trp66 is a conserved heme pocket residue implicated in heme ruffling. Mutant is inactive |
-, 740703 |
