EC Number   |
Protein Variants |
Reference |
|---|
  1.14.20.7 | A198V |
site-directed mutagenesis, the mutant produces large amounts of L-DELTA1-pyrroline-5-carboxylate but very little ethylene |
742750 |
| 1.14.20.7 | A198V |
the mutant shows about 60% of wild type activity |
742750 |
| 1.14.20.7 | A199G |
the mutant shows reduced activity compared to the wild type enzyme |
742191 |
| 1.14.20.7 | A218R |
inactive |
742750 |
| 1.14.20.7 | A218V |
the mutant shows about 3% of wild type activity |
742750 |
| 1.14.20.7 | A281V |
site-directed mutagenesis, the mutant produces low levels of products in comparison to the wild-type enzyme |
742750 |
| 1.14.20.7 | C317A |
the mutant shows about 34% of wild type activity |
743692 |
| 1.14.20.7 | C317S |
the mutant shows about 21% of wild type activity |
743692 |
| 1.14.20.7 | D191E |
the D191E variant degrades L-Arg and 2-oxoglutarateto pyrroline-5-carboxylate (again detected after reduction to proline and Fmoc derivatization) and succinate nearly stoichiometrically, with only about 5% of the cosubstrate being fragmented to ethylene |
764978 |
| 1.14.20.7 | E213A |
the mutant shows about 90% of wild type activity |
742750 |
