EC Number   |
Protein Variants |
Reference |
|---|
   1.14.20.1 | A106T |
80% relative activity with 1 mM substrate and 170% relative activity with 10 mM penicillin G compared to wild-type activity detected with 1 mM penicillin G, 1.5fold reduction in Km, penicillin G as substrate |
671443 |
| 1.14.20.1 | A106T/C155Y |
epPCR random mutagenesis |
671443 |
| 1.14.20.1 | A106T/M188V |
epPCR random mutagenesis |
671443 |
| 1.14.20.1 | A11V/T91A/C281Y/I305L |
220% relative activity with 1 mM and 200% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G |
671443 |
| 1.14.20.1 | A61E |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type |
724066 |
| 1.14.20.1 | C155Y |
90% relative activity with 1 mM and 180% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G, 1.5fold reduction in Km, penicillin G as substrate |
671443 |
| 1.14.20.1 | C155Y |
site-directed mutagenesis |
724024 |
| 1.14.20.1 | C155Y |
the mutant has 1.5fold increment in kcat/Km value when compared with the wild type enzyme |
699366 |
| 1.14.20.1 | C155Y/Y184H/S261M/V275I/C281Y/I305M |
site-directed mutagenesis, the mutant shows 87fold increased kcat/Km for penicillin G compared to the wild-type |
-, 745495 |
| 1.14.20.1 | C155Y/Y184H/V275I/C281Y |
580% relative activity with 1 mM and 670% relative activity with 10 mM substrate penicillin G compared to wild-type activity detected with 1 mM penicillin G |
671443 |
