EC Number   |
Protein Variants |
Reference |
|---|
   1.14.19.35 | C103Y |
mutation occuring in the fad3 fad7 fad8 triple mutant |
-, 734740 |
| 1.14.19.35 | more |
constitutive expression of the fad8 cDNA in transgenic plants of a fad7 mutant JBl01, via Agrobacterium turnefaciens C58 (pGV3101) containing plasmid pBI/fad8 or pBIl2l, results in genetic complementation of the mutation, indicating that the fad7 and fad8 gene products are functionally equivalent. Expression of the fad8 cDNA in transgenic plants often results in the co-suppression of both the endogenous fad7 and fad8 genes in spite of the fact that these two genes share only about 75% nucleotide identity |
-, 734945 |
| 1.14.19.35 | more |
construction of a fad7/fad8 double mutant with reduced overall levels of trienoic fatty acids in leaf tissues. A series of FAD7-FAD8 chimeric genes, each encoding a functional plastidial omega-3 desaturase, are introduced into the Arabidopsis thaliana fad7/fad8 double mutant, structures, overview. All transformants show an unaltered temperature response, and none of the transformants exhibit an exceptional phenotype |
-, 734153 |
| 1.14.19.35 | more |
construction of a insertional mutant fad7i (SALK_147096C) inactivation mutant of FAD7, analysis of the fatty acid composition of the fad7i mutant shows compensatory responses at the TA level as a result of inactivation of the FAD7 gene, absence of AtFAD7 transcript in the mutant. Contruction of a fad7/fad8 double knockout mutant. Given the strong reduction (70%) in 18:3 content observed in the fad7/fad8 double mutant, in mutant fad7i after disruption of the AtFAD7 gene, FAD8 enzymatic activity is able to maintain, at least partially (43.7%), the amount of 18:3 and to a much lesser extent that of 16:3 (23.2%) at 22°C. Mutant genotypes and phenotypes, overview. Temperature-dependent expression levels. Disruption of the AtFAD7 gene in the insertion mutant does not result in significant changes in the transcript levels of FAD7 |
-, 745809 |
| 1.14.19.35 | more |
construction of a tandem T-DNA FAD7 insertion omega-3 desaturase mutant line SALK_147096C/fad7i. Construction of double fad7/fad8 and triple fad3/fad7/fad8 mutants. Fatty acid composition of total leaf lipids from wild-type and the different mutant lines grown at 22°C, overview. Mutant fad7i maintains 74% of 18:3 production with respect to Col-0 while only 23% of 16:3 synthesis is maintained in this mutant. The fad7/fad8 double mutant also shows a considerable reduction in trienoic fatty acids, particularly in 16:3, which is undetectable |
-, 734740 |
| 1.14.19.35 | more |
construction of a tandem T-DNA FAD8 insertion omega-3 desaturase mutant line SALK_093590 with no phenotype at 22°C. Construction of double fad7/fad8 and triple fad3/fad7/fad8 mutants. FAD8-YFP overexpressing lines show a specific increase in 18:3 fatty acids at 22°C. Fatty acid composition of total leaf lipids from wild-type and the different mutant lines grown at 22°C, overview. Disruption of the AtFAD8 gene in mutant fad8i results in trienoic fatty acid levels almost similar to those from wild-type Col-0. The fad7/fad8 double mutant also shows a considerable reduction in trienoic fatty acids, particularly in 16:3, which is undetectable |
-, 734740 |
| 1.14.19.35 | more |
generation of knockout mutants of gene OsFAD8, fatty acids contents (mol%) in leaves from wild-type rice and osfad8 mutant lines at 4°C and 28°C, overview |
746126 |
| 1.14.19.35 | more |
generation of the fad7 knockout mutant line JB101 |
-, 734986 |
| 1.14.19.35 | more |
mutation of Thr286 may directly affect the structure of the transmembrane domain of the enzyme |
733913 |
| 1.14.19.35 | more |
recombinant overexpression of FAD8 increases tolerance to drought in tobacco plants and to osmotic stress in cultured cells. Ectopic overexpression of FAD8 induces an increased ratio mainly in the plastidic lipids |
-, 734907 |
