EC Number   |
Protein Variants |
Reference |
|---|
    1.14.15.33 | D50N |
increase in catalytic activity. Binding affinity for 10-desoxymethymycin increases about 4fold, binding affinity for narbomycin about 1.3fold compared to wild-type. 60% conversion of 10-desoxymethymycin, ratio of products neomethymycin to methymycin is 1.15. 44.4% conversion of substrate narbomycin |
727865 |
| 1.14.15.33 | D50N |
the mutant enzyme provides more efficient hydroxylation of both macrolide substrates when compared with native enzyme |
725370 |
| 1.14.15.33 | D50N/E85Q |
18.4% conversion of 10-desoxymethymycin, ratio of products neomethymycin to methymycin is 0.38. 3.5% conversion of substrate narbomycin |
727865 |
| 1.14.15.33 | D50N/E94Q |
no conversion of 10-desoxymethymycin. 5.3% conversion of substrate narbomycin |
727865 |
| 1.14.15.33 | E85A/E94A |
the mutant enzyme is catalytically incompetent toward both endogenous macrolactone substrates |
725370 |
| 1.14.15.33 | E85Q |
18% conversion of 10-desoxymethymycin, ratio of products neomethymycin to methymycin is 0.26. 8.1% conversion of substrate narbomycin |
727865 |
| 1.14.15.33 | E85Q |
change in product profile, mutation shifts the product ratio of 10-deoxymethymycin hydroxylation toward methymycin |
725370 |
| 1.14.15.33 | E94Q |
no conversion of 10-desoxymethymycin. 5.4% conversion of substrate narbomycin |
727865 |
