EC Number |
Protein Variants |
Reference |
---|
1.14.13.50 | F223A |
site-directed mutagenesis, increased activity compared to the wild-type enzyme |
658439 |
1.14.13.50 | F225A |
site-directed mutagenesis, increased activity compared to the wild-type enzyme |
658439 |
1.14.13.50 | F85A |
site-directed mutagenesis, 60% decreased activity compared to the wild-type enzyme |
658439 |
1.14.13.50 | F85A |
site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme |
658210 |
1.14.13.50 | F87A |
site-directed mutagenesis, mutant enzyme is nearly not soluble in contrast to the wild-type, highly reduced activity compared to the wild-type enzyme |
658439 |
1.14.13.50 | moe |
construction of an allelic exchange mutant and a pcpD knockout mutant strains, the latter show reduced activity at high pentachlorophenol concentrations, not at low concentrations |
659022 |
1.14.13.50 | more |
introduction of tobacco etch virus protease cleavage site into the enzyme as a glutathione-S-transferase fusion protein |
438820 |
1.14.13.50 | more |
targeted mutagenesis via allelic exchange, construction of isogenic mutant without PCP 4-monooxygenase activity and 2 cointegration mutants with slow activity |
438819 |
1.14.13.50 | R235A |
site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme |
658210 |
1.14.13.50 | R235E |
site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme |
658210 |