EC Number   |
Protein Variants |
Reference |
|---|
    1.14.12.23 | A405G |
synthesizes 17% 3-methylcatechol, 83% 4-methylcatechol from substrate 3-nitrotoluene |
-, 733176 |
| 1.14.12.23 | F293H |
mutation in alpha-subunit of oxygenase, residue is important for determining regiospecificity with nitroarene substrates and enantiospecificity with naphthalene |
733154 |
| 1.14.12.23 | F293I |
mutation in alpha-subunit of oxygenase, residue is important for determining regiospecificity with nitroarene substrates and enantiospecificity with naphthalene |
733154 |
| 1.14.12.23 | F293Q |
mutation in alpha-subunit of oxygenase, residue is important for determining regiospecificity with nitroarene substrates and enantiospecificity with naphthalene. Mutant is 2.5 times faster than wild-type at oxidizing 2,6-dinitrotoluene while retaining a similar Km for the substrate |
733154 |
| 1.14.12.23 | F350T |
contrary to wild-type, mutant produces 3-methyl-4-nitrocatechol from 2,6-dinitrotoluene and 3-amino-4-methyl-5-nitrocatechol and 2-amino-4,6-dinitrobenzyl alcohol from 2-amino-4,6-dinitrotoluene, and mutant releases nitrite from 2,3-dinitrotoluene sixfold faster than wild-type |
-, 734066 |
| 1.14.12.23 | F350T/G407S |
double mutant increases the rate of oxidation of 2,3-dinitrotoluene, 2,6-dinitrotoluene , and 2-amino-4,6-dinitrotoluene threefold relative to variant F350T |
-, 734066 |
| 1.14.12.23 | G50S/L225R/A269T |
mutant displays increased rate of 4-amino-2-nitrotoluene oxidation and additionally produces oxidation product 4-amino-2-nitrocresol (enhanced 11fold relative to wild-type) as well as 4-amino-2-nitrobenzyl alcohol |
-, 734066 |
| 1.14.12.23 | I204A |
synthesizes 2% 3-methylcatechol, 97% 4-methylcatechol and 1% 3-nitrobenzyl alcohol from substrate 3-nitrotoluene |
-, 733176 |
| 1.14.12.23 | I204T |
synthesizes 5% 3-methylcatechol, 93% 4-methylcatechol and 2% 3-nitrobenzyl alcohol from substrate 3-nitrotoluene |
-, 733176 |
| 1.14.12.23 | I204V |
synthesizes 13% 3-methylcatechol, 83% 4-methylcatechol and 5% 3-nitrobenzyl alcohol from substrate 3-nitrotoluene |
-, 733176 |
