EC Number |
Protein Variants |
Reference |
---|
1.14.11.8 | D244E |
the Km-value of the variant enzyme for N6,N6,N6-trimethyl-L-lysine is 3.7fold lower than the Km-value of the wild-type enzyme, the Km-value of the variant enzyme for 2-oxoglutarate is 2.7fold higher than the Km-value of the wild-type enzyme |
764104 |
1.14.11.8 | H389L |
enzyme not functional |
675029 |
1.14.11.8 | more |
construction of senescence marker protein-30/gluconolactonase knockout mice, which cannot synthesize L-ascorbate in vivo, the mutant mice nevertheless produce normal levels of carnitine when fed an L-ascorbate lacking diet |
691166 |
1.14.11.8 | T269A |
the Thr269Ala variant displays high enzymatic activity (96% at 0.10 mM, 76% at 0.003 mM) for the conversion of (2S)-Nepsilon-trimethyllysine to (2S,3S)-3-hydroxy-Nepsilon-trimethyllysine |
764104 |
1.14.11.8 | W221F |
the variant displays considerably reduced enzymatic activity (32%), implying that the OH group of Tyr404 contributes to stronger interaction with the trimethylammonium group of (2S)-Nepsilon-trimethyllysine |
764104 |
1.14.11.8 | Y217D |
the variants does not display any enzymatic activity within limits of detection |
764104 |
1.14.11.8 | Y217E |
the Thr269Ala variant displays high enzymatic activity (96% at 0.10 mM, 76% at 0.003 mM) for the conversion of (2S)-Nepsilon-trimethyllysine to (2S,3S)-3-hydroxy-Ne-trimethyllysine |
764104 |