EC Number |
Protein Variants |
Reference |
---|
1.14.11.69 | D191A |
site-directed mutagenesis, the mutant shows about 95%reduced activity with H3K9me3 compared to wild-type, and no activity with H3K36me3 |
754871 |
1.14.11.69 | G133A |
site-directed mutagenesis, the mutation in the catalytic domain abrogates enzyme activity |
753371 |
1.14.11.69 | G138A |
site-directed mutagenesis, the mutation in the catalytic domain abrogates enzyme activity |
753371 |
1.14.11.69 | G165A |
site-directed mutagenesis, the mutation in the catalytic domain abrogates enzyme activity |
753371 |
1.14.11.69 | G170A |
site-directed mutagenesis, the mutation in the catalytic domain abrogates enzyme activity |
753371 |
1.14.11.69 | H195A |
construction of a mutant dKDM4A in which a conserved amino acid in the iron-binding site is mutated to alanine, the mutant dKDM4A has no demethylation activity on histones H3K36me3 and H3K36me2 |
700209 |
1.14.11.69 | H195A |
site-directed mutagenesis |
724813 |
1.14.11.69 | H195A |
site-directed mutagenesis, mutation of an Fe2+ binding residue, abolishes demethylase activity of dJMJD2(1)/CG15835 |
754921 |
1.14.11.69 | H235A |
catalytically deficient mutant |
-, 726071, 754922 |
1.14.11.69 | more |
construction of a P-element insertion mutant of dKDMA4, the mutant is homozygous viable, the P element insertion elevates the bulk level of histone H3K36me3 in mutant embryos. Overexpression of dKDM4A seems to only lead to demethylation of histone H3K36, since the level of histone H3K9me3 and H3K4me2 remains unchanged |
700209 |