EC Number   |
Protein Variants |
Reference |
|---|
   1.14.11.56 | D108G |
remodeling of L-proline cis-4-hydroxylase into a halogenase. Halogenation of L-proline exclusively occurs at the C3-position, the retained hydroxylation activity leads to derivatization at the C4-position |
764490 |
| 1.14.11.56 | V95A |
no increase in hydroxylation activity nor any improvement in the cis-5/cis-3 product ratio |
-, 739780 |
| 1.14.11.56 | V95A |
site-directed mutagenesis, the mutant does not show any increase in hydroxylation activity nor any improvement in the cis-5/cis-3 ratio compared to the wild-type enzyme |
743931 |
| 1.14.11.56 | V95A/V97A |
no increase in hydroxylation activity nor any improvement in the cis-5/cis-3 product ratio |
-, 739780 |
| 1.14.11.56 | V95A/V97A |
site-directed mutagenesis, the mutant does not show any increase in hydroxylation activity nor any improvement in the cis-5/cis-3 ratio compared to the wild-type enzyme |
743931 |
| 1.14.11.56 | V95W |
mutation significantly reduces the amount of cis-3-hydroxy-L-pipecolic acid (<5%) production compared with that of cis-5-hydroxy-L-pipecolic acid |
739780 |
| 1.14.11.56 | V97A |
2.5fold increase in production of cis-5-hydroxy-L-pipecolic acid, ratio of cis-5/cis-3 products is similar to wild-type |
-, 739780 |
| 1.14.11.56 | V97A |
site-directed mutagenesis, the mutant shows increased activity and production of cis-4-hydroxy-L-proline |
743931 |
| 1.14.11.56 | V97C |
4fold increase in production of cis-5-hydroxy-L-pipecolic acid, ration of §-cis/5cis products is similar to wild-type |
-, 739780 |
| 1.14.11.56 | V97C |
site-directed mutagenesis |
743931 |
