EC Number |
Protein Variants |
Reference |
---|
1.14.11.21 | C162L |
isozyme CAS2, site-directed mutagenesis, same activity in hydroxylation of N-alpha-acetyl-L-arginine as the wild-type enzyme |
639283 |
1.14.11.21 | C200L |
isozyme CAS2, site-directed mutagenesis, 42% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme |
639283 |
1.14.11.21 | C201L |
isozyme CAS2, site-directed mutagenesis, 40% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme |
639283 |
1.14.11.21 | C8L |
isozyme CAS2, site-directed mutagenesis, 42% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme |
639283 |
1.14.11.21 | H109L |
isozyme CAS2, site-directed mutagenesis, 86% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme |
639283 |
1.14.11.21 | H109Q |
isozyme CS2, site-directed mutagensis, 83% activity of clavaminate formation compared to the wild-type enzyme, unaltered hydroxylating activity |
639282 |
1.14.11.21 | H122L |
isozyme CAS2, site-directed mutagenesis, below 1% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme |
639283 |
1.14.11.21 | H122Q |
isozyme CS2, site-directed mutagensis, 96% activity of clavaminate formation compared to the wild-type enzyme, unaltered hydroxylating activity |
639282 |
1.14.11.21 | H131L |
isozyme CAS2, site-directed mutagenesis, 36% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme |
639283 |
1.14.11.21 | H131Q |
isozyme CS2, site-directed mutagensis, 16% activity of clavaminate formation compared to the wild-type enzyme, unaltered hydroxylating activity |
639282 |