EC Number |
Protein Variants |
Reference |
---|
1.13.12.13 | A33N |
site-directed mutagenesis, mutation of the catalytic subunit, the mutant shows increased bioluminesce intensity compared to the wild-type |
744220 |
1.13.12.13 | A4E |
site-directed mutagenesis, mutation of the catalytic subunit, the mutant shows wild-type bioluminesce intensity |
744220 |
1.13.12.13 | A4E/Q11R/A33K/V44I/A54F/P115E/Q124K/Y138I |
mutant is 29000fold brighter than mutant N166R. Western blot analysis shows that mutant A4E/Q11R/A33K/V44I/A54F/P115E/Q124K/Y138I is produced more efficiently than mutant N166R in cells. The increased expression is consistent with improved enzyme stability at 37°C, where the half-life of activity retention is increased 65fold over that of mutant N166R |
727290 |
1.13.12.13 | A4E/Q11R/A33K/V44I/A54F/P115E/Q124K/Y138I/Q18L/F54I/F68Y/L72Q/M75K/I90V/L27V/K33N/K43R/Y68D |
mutant Nluc: Nluc paired with furimazine produces 2.5 millionfold brighter luminescence in mammalian cells relative to Oluc-19 with coelenterazine. The luminescence produced by Nluc decays with a half-life more than 2 h, significantly longer than for mutant A4E/Q11R/A33K/V44I/A54F/P115E/Q124K/Y138I. Nluc increased luminescence is gained mostly through improvements in protein stability, where Nluc shows markedly greater retention of activity in lysates following incubation at 37°C |
727290 |
1.13.12.13 | A4E/Q11R/Q18L/L27V/A33N/K43R/V44I/A54I/F68D/L72Q/M75K/I90V/P115E/Q124K/Y138I/N166R |
construction of mutant nanoKAZ, a mutant with 16 amino acid substitutions of the catalytic subunit, by site-directed mutagenesis, the mutant nanoKAZ lacks the amino-terminal signal peptide, crystal structure determination and analysis, structure-function relationship in nanoKAZ, overview. The truncation of 10 amino acid residues at N- and C-terminal regions of nanoKAZ causes a complete loss of luminescence activity. Both the alpha1-helix and beta11-strand in the nanoKAZ molecule might serve to stabilize the molecule, which is essential in catalyzing the luminescence reaction |
744231 |
1.13.12.13 | A54I |
site-directed mutagenesis, mutation of the catalytic subunit, the mutant shows increased bioluminesce intensity compared to the wild-type |
744220 |
1.13.12.13 | A54I/Y138I |
site-directed mutagenesis, mutation of the catalytic subunit, the mutant shows increased bioluminesce intensity compared to the wild-type |
744220 |
1.13.12.13 | C164A |
mutation does not significantly affect catalytic activity of subunit kOLase |
689868 |
1.13.12.13 | C164G |
mutation does not significantly affect catalytic activity of subunit kOLase |
689868 |
1.13.12.13 | C164S |
mutation does not significantly affect catalytic activity of subunit kOLase |
689868 |