EC Number   |
Protein Variants |
Reference |
|---|
    1.12.99.6 | A204F |
no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type |
659355 |
| 1.12.99.6 | C176A |
the Cys176 sulfur and unknown ligands of the iron complex of the wild-type enzyme are replaced by the dithiothreitol present in the crystallization solution |
703728 |
| 1.12.99.6 | C250A |
reduced activity |
690060 |
| 1.12.99.6 | C81S |
theoretical 3D strucutural model. For the wild-type, the hydrogen bond of the network involving H82 and the bridging cysteines is formed with the sulfur atom of C78 whereas for the C81S mutant, it is formed with the bridging sulfur atom from C600. Calculations indicate a water molecule close to C81, which influences the IR spectra |
-, 726107 |
| 1.12.99.6 | D100N |
no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type |
659355 |
| 1.12.99.6 | D202V/K492 |
variant epHycE70, has 11fold higher hydrogen production and 7fold higher hydrogen yield from formate compared to wild-type |
684648 |
| 1.12.99.6 | D210N/I271F/K545R |
variant epHycE23-2, has 8fold higher hydrogen production and 4fold higher hydrogen yield from formate compared to wild-type |
684648 |
| 1.12.99.6 | D88N |
no difference in activity with 2,3-dimethyl1,4-naphthoquinone and benzyl viologen to wild-type |
659355 |
| 1.12.99.6 | E25D |
approx. 50% of wild-type H2 uptake activity |
659310 |
| 1.12.99.6 | E25Q |
less than 0.1% of wild-type H2 uptake activity |
659310 |
