EC Number |
Protein Variants |
Reference |
---|
1.11.1.12 | C2S/C10S/C37S/C66S/C75S/C107S/C148S |
substitution of all Cys residues by Ser. The mutant exhibits a Ping-Pong mechanism and structure similar to that of native GPx4, the activity is similar to wild-type |
742558 |
1.11.1.12 | C66S |
mutation of the second reactive Cys residue, crystallization data |
763834 |
1.11.1.12 | C82S |
in the presence of thioredixin system, wild-type Hyr1 can consume H2O2 rapidly, while the C82S mutant totally abolish the activity, indicating the important role of Cys82 in Trx2 specificity |
701118 |
1.11.1.12 | more |
(RBL)2H3 cell line stably overexpressing mitochondrial phospholipid hydroperoxide glutathione peroxidase from transfected expression plasmid |
394883 |
1.11.1.12 | more |
deletion mutants of gpx1delta, gpx2delta and gpx3delta showing diminished enzyme activity, triple mutant gpx1delta/gpx2delta/gpx3delta has almost no remaining activity |
394874 |
1.11.1.12 | more |
enzyme silencing of glutathione peroxidase 4 by shRNA hairpin oligonucleotides, designed by selecting an 18-nt site from the human GPx4 complete mRNA (NCBI Accession: BC046163.1) optimized for siRNA targeting of GPx4 mRNA (S080) or a random 18-nt sequence (scramble) with no predicted homology to human genomic or transcript sequences. The cloning strategy nests the shRNA fragments between 5' and 3' miRNA30 adaptors within the 3' untranslated region of a polycistronic red fluorescent protein reporter gene under the control of a CMV promoter. Stable expression of the GPx4 knockdown, and stable expression of genetically encoded fluorescent reporters attained via lentiviral transduction of BEAS-2B cells. Lentivirus encoding roGFP2 or HyPer is incubated for 4 hr with wild-type BEAS-2B cells in keratinocyte basal media (KBM) using a multiplicity of infection of 2-5 |
764204 |
1.11.1.12 | more |
replication-deficient human adenovirus containing rat GPX4 cDNA (adenovirus GPX4 [Ad-GPX4]) is used to overexpress GPX4 in rats by injection into the right lateral ventricle of the brain of male Sprague-Dawley rats |
-, 765297 |
1.11.1.12 | more |
silencing of GPx4 by RNA interference, siRNA-mediated knockdown of GPx4 in insulin-producing beta-cells. Overexpression of GPx4 in insulin-producing RINm5F cells prevents tert-BHP-induced cell death. GPx4 overexpression provides minor protection of insulin-producing RINm5F cells against pro-inflammatory cytokines |
-, 764207 |
1.11.1.12 | more |
substitution of residues Cys2, Cys37, Cys75, Cys107, and Cys148 with Sec leads to loss of activity. The catalytic activity of GPx4 mutants increases as the number of noncatalytic Sec residues decreases |
742558 |
1.11.1.12 | Q81A |
no residual activity |
764227 |