EC Number   |
Protein Variants |
Reference |
|---|
   1.1.5.5 | A26V |
random mutagenesis, the mutation has no effect on PQQ-ADH activity and ethanol oxidizing ability |
-, 725154 |
| 1.1.5.5 | F412I/W561Q |
wild-type enzyme shows no activity with 5-(hydroxymethyl)furfural, mutant enzyme shows low activity (0.35 U/mg) |
762532 |
| 1.1.5.5 | F412I/W561S |
wild-type enzyme shows no activity with 5-(hydroxymethyl)furfural, mutant enzyme shows low activity (0.12 U/mg) |
762532 |
| 1.1.5.5 | F412V/W561A |
wild-type enzyme shows no activity with 5-(hydroxymethyl)furfural, mutant enzyme shows activity (1.04 U/mg). The mutant enzyma slso The KM-value for ethanol is approximately 525fold higher than that the wild-type value, whereas the maximal velocity is unchanged. This suggests that the oxidative capability of the PedHF412V/W561A variant is unaffected by the amino acid changes, and only the substrate binding is modulated |
762532 |
| 1.1.5.5 | G55D |
random mutagenesis, the mutation has no effect on PQQ-ADH activity and ethanol oxidizing ability |
-, 725154 |
| 1.1.5.5 | L18Q |
random mutagenesis, the mutation has no effect on PQQ-ADH activity and ethanol oxidizing ability |
725154 |
| 1.1.5.5 | more |
construction of enzyme electrodes containing pyrroloquinoline quinone-dependent alcohol dehydrogenase as a biological component in combination with 4-ferrocenylphenol as an electron transfer mediator between PQQ and a carbon electrode for measurements of ethanol, overview. The biosensor shows the highest response at pH 5.5 and the working potential of 0.3 V, versus AgNAgCl, for ADH |
-, 686376 |
| 1.1.5.5 | more |
disruption of genes exaA2 and exaA3 |
-, 726014 |
| 1.1.5.5 | more |
random mutagenesis of adhS gene, complete loss of PQQ-ADH activity and ethanol oxidizing ability are observed in the mutants lacking of the 140 and 73 amino acid residues at the C-terminal, whereas the lack of 22 amino acid residues at the C-terminal affected neither the PQQ-ADH activity nor ethanol oxidizing ability |
-, 725154 |
| 1.1.5.5 | T104K |
random mutagenesis, the mutation leads to complpete loss of ethanol oxidizing ability |
725154 |
