EC Number   |
Protein Variants |
Reference |
|---|
   1.1.3.B5 | G392F |
mutant does not display measurable activity |
763480 |
| 1.1.3.B5 | I427A |
mutant is significantly more efficient with 2,6-dimethoxy-4-allylphenol than wild-type |
762539 |
| 1.1.3.B5 | I427T |
mutant does not display measurable activity |
763480 |
| 1.1.3.B5 | L381W |
mutant does not display measurable activity |
763480 |
| 1.1.3.B5 | L438C |
variant displays lowered activity towards all substrates as compared to wild-type |
763480 |
| 1.1.3.B5 | M282L |
substrate specificity profile is similar to wild-type |
763480 |
| 1.1.3.B5 | more |
exchange of a loop at the dimer-dimer interface in octameric vanillin oxidase that is not present in dimeric EUGO. A vanillin oxidase variant where the loop was deleted, loopless VAO, exclusively forms dimers. Introduction of the loop into EUGO is not sufficient to induce its octamerization. Neither variant displays major changes in its catalytic properties as compared to the wild-type enzyme |
763012 |
| 1.1.3.B5 | Q425T |
display similar activity to the wild-type enzyme with vanillyl alcohol, but no measurable activity towards any other substrate |
763480 |
| 1.1.3.B5 | V436I |
variant displays lowered activity towards vanillyl alcohol and eugenol |
763480 |
