EC Number   |
Protein Variants |
Reference |
|---|
  1.1.1.B66 | H130A |
site-directed mutagenesis, the mutant shows highly reduced activity with substrate (4E)-5-carbamimidamido-2-iminopent-4-enoic acid, but unaltered activity with 5-carbamimidamido-2-iminopentanoic acid compared to wild-type |
763186 |
| 1.1.1.B66 | H130A/Y132F |
site-directed mutagenesis, the mutant shows highly reduced activity with substrate (4E)-5-carbamimidamido-2-iminopent-4-enoic acid, but increased activity with 5-carbamimidamido-2-iminopentanoic acid compared to wild-type |
763186 |
| 1.1.1.B66 | more |
the mutations do not impact dimerization |
763186 |
| 1.1.1.B66 | R229A |
site-directed mutagenesis, the mutant shows reduced activity with substrates (4E)-5-carbamimidamido-2-iminopent-4-enoic acid and 5-carbamimidamido-2-iminopentanoic acid compared to wild-type |
763186 |
| 1.1.1.B66 | Y132F |
site-directed mutagenesis, the mutant shows highly reduced activity with substrate (4E)-5-carbamimidamido-2-iminopent-4-enoic acid, but unaltered activity with 5-carbamimidamido-2-iminopentanoic acid compared to wild-type |
763186 |
| 1.1.1.B66 | Y97F |
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme |
763186 |
