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EC Number Protein Variants Commentary Reference
Display the reaction diagram Show all sequences 1.1.1.B19more engineered Sacchyromyces cerevisiae expressing NADPH-linked xylose reductase (XR) and NAD+-linked xylitol dehydrogenase (XDH) produces substantial amounts of the reduced byproducts under anaerobic conditions due to the cofactor difference of XR and XDH. While the additional expression of a water-forming NADH oxidase (NoxE) from Lactococcus lactis in engineered Saccharomyces cerevisiae with the XR/XDH pathway leads to reduced glycerol and xylitol production and increased ethanol yields from xylose, volumetric ethanol productivities by the engineered yeast decrease because of growth defects from the overexpression of noxE. High cell density inoculum for xylose fermentation by strain SR8 expressing noxE, resulting in strain SR8N, shows a higher ethanol yield and lower byproduct yields, and also exhibits a high ethanol productivity during xylose fermentation. Growth defects from noxE overexpression can be overcome in the case of fermenting lignocellulose-derived sugars such as glucose and xylosen -, 743034
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