EC Number |
Protein Variants |
Reference |
---|
1.1.1.9 | D202A/L203R/V204S/E205P/S206R |
site-directed mutagenesis, introduction of multiple site-directed mutations in the coenzyme-binding pocket of Galactocandida mastotermitis XDH to enable activity with NADP+, which is lacking in the wild-type enzyme, genetic metabolic engineering for improvement of xylose metabolism and fermentation in wild-type Saccharomyces cerevisiae strains, which are not able to naturally metabolize D-xylulose, overview |
696889 |
1.1.1.9 | D205A |
site-directed mutagenesis, coenzyme preference of the mutant RpXDH is partially reversed from NAD+ to NADP+ |
-, 742112 |
1.1.1.9 | D205A/I206R |
site-directed mutagenesis, coenzyme preference of the mutant RpXDH is reversed from NAD+ to NADP+ |
-, 742112 |
1.1.1.9 | D205A/I206R |
the mutant shows cofactor preference for NADP+ |
-, 737866 |
1.1.1.9 | D207A |
kcat/Km for NAD+ is 3.6fold lower than wild-type value, kcat/Km for NADP+ is 4.3fold higher than wild-type value |
669305 |
1.1.1.9 | D207A/F209S |
kcat/Km for NAD+ is 2.2fold lower than wild-type value, kcat/Km for NADP+ is 745fold higher than wild-type value |
669305 |
1.1.1.9 | D207A/I208R |
kcat/Km for NAD+ is 2.5fold lower than wild-type value, kcat/Km for NADP+ is 229fold higher than wild-type value |
669305 |
1.1.1.9 | D207A/I208R/F209S |
kcat/Km for NAD+ is 15.2fold lower than wild-type value, kcat/Km for NADP+ is 4292fold higher than wild-type value, increased thermostability |
669305 |
1.1.1.9 | D207A/I208R/F209S |
mutant bearing a reversal of coenzyme specificity from NAD+ to NADP+ is introduced into Saccharomyces cerevisiae. kcat/Km (NAD+): 181 l/min/mmol, kcat/Km (NADP+): 2790 l/min/mmol, Km (xylitol in the presence of NAD+): 31.1 mM, NADP+-dependent activity: 0.782 U/mg, NAD+-dependent activity: 0.271 U/mg. In xylose fermentation a large decrease in xylitol and glycerol yield is shown, while the xylose consumption and ethanol yield are decreased |
688749 |
1.1.1.9 | D207A/I208R/F209S/N211R |
kcat/Km for NAD+ is 32.9fold lower than wild-type value, kcat/Km for NADP+ is 4292fold higher than wild-type value, increased thermostability |
669305 |